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plasma non esterified fatty acid nefa concentrations  (Randox)
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Effects of 12 weeks of high-fat diet (HFD) or normal diet (ND) on oral glucose tolerance test (OGTT) and insulin responses. (A) OGTT results. (B) The plasma insulin levels were determined during the OGTT. (C) Area under the curve (AUC) of the time-blood glucose curve of OGTT. (D) AUC of the time-blood insulin curve of OGTT. (E) Glycated hemoglobin (HbA1c; %). (F) Serum triglyceride levels. (G) <t>Serum</t> <t>non-esterified</t> fatty acid <t>(NEFA)</t> levels in different groups of mice. KI, knock-in; WT, wild-type; NS, not significant. a P <0.05; b P <0.01; c P <0.001; d P <0.05; e P <0.001; f P <0.0001.
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nefa concentrations  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
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Nanjing Jiancheng Bioengineering Research Institute Co Ltd nefa concentrations
Effects of 12 weeks of high-fat diet (HFD) or normal diet (ND) on oral glucose tolerance test (OGTT) and insulin responses. (A) OGTT results. (B) The plasma insulin levels were determined during the OGTT. (C) Area under the curve (AUC) of the time-blood glucose curve of OGTT. (D) AUC of the time-blood insulin curve of OGTT. (E) Glycated hemoglobin (HbA1c; %). (F) Serum triglyceride levels. (G) <t>Serum</t> <t>non-esterified</t> fatty acid <t>(NEFA)</t> levels in different groups of mice. KI, knock-in; WT, wild-type; NS, not significant. a P <0.05; b P <0.01; c P <0.001; d P <0.05; e P <0.001; f P <0.0001.
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Effects of 12 weeks of high-fat diet (HFD) or normal diet (ND) on oral glucose tolerance test (OGTT) and insulin responses. (A) OGTT results. (B) The plasma insulin levels were determined during the OGTT. (C) Area under the curve (AUC) of the time-blood glucose curve of OGTT. (D) AUC of the time-blood insulin curve of OGTT. (E) Glycated hemoglobin (HbA1c; %). (F) Serum triglyceride levels. (G) Serum non-esterified fatty acid (NEFA) levels in different groups of mice. KI, knock-in; WT, wild-type; NS, not significant. a P <0.05; b P <0.01; c P <0.001; d P <0.05; e P <0.001; f P <0.0001.

Journal: Endocrinology and Metabolism

Article Title: Reduced Sphingosine-1-Phosphate Levels Exacerbate Type 2 Diabetes Mellitus and Associated Complications in a High-Fat Diet Mouse Model

doi: 10.3803/EnM.2025.2360

Figure Lengend Snippet: Effects of 12 weeks of high-fat diet (HFD) or normal diet (ND) on oral glucose tolerance test (OGTT) and insulin responses. (A) OGTT results. (B) The plasma insulin levels were determined during the OGTT. (C) Area under the curve (AUC) of the time-blood glucose curve of OGTT. (D) AUC of the time-blood insulin curve of OGTT. (E) Glycated hemoglobin (HbA1c; %). (F) Serum triglyceride levels. (G) Serum non-esterified fatty acid (NEFA) levels in different groups of mice. KI, knock-in; WT, wild-type; NS, not significant. a P <0.05; b P <0.01; c P <0.001; d P <0.05; e P <0.001; f P <0.0001.

Article Snippet: Plasma non-esterified fatty acid (NEFA) concentrations were measured using the Randox NEFA assay kit (Randox Laboratories, Antrim, UK; #FA 115) following the manufacturer’s instructions.

Techniques: Clinical Proteomics, Knock-In